Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural requirements for alpha-mating factor activity

G Houen1, O Nielsen, C Flanagan

  • 1Department of Autoimmunology, Statens Seruminstitut, Copenhagen, Denmark.

FEBS Letters
|August 5, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virus antibody responses in patients with myelin oligodendrocyte glycoprotein antibody-associated disease and multiple sclerosis.

Multiple sclerosis and related disorders·2026
Same author

Systemic antibiotic use in fire-affected koalas (Phascolarctos cinereus) admitted to two wildlife treatment facilities during the 2019-2020 wildfires.

Australian veterinary journal·2025
Same author

"They just looked at me like I was human": The experiences of parenting women and providers with substance use disorder treatment.

Journal of substance use and addiction treatment·2023
Same author

Antibodies to calnexin and mutated calreticulin are common in human sera.

Current research in translational medicine·2023
Same author

Virus-specific antibody indices may supplement the total IgG index in diagnostics of multiple sclerosis.

Journal of neuroimmunology·2022
Same author

Role and Function of the Clinical Tutor in Mental Health Nursing in Ireland.

Issues in mental health nursing·2021
Same journal

Regulation of CFTR stability at the plasma membrane-Mechanisms and therapeutic opportunities in cystic fibrosis.

FEBS letters·2026
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
See all related articles

Luteinizing hormone-releasing hormone (LHRH) does not exhibit alpha-mating factor (alpha-MF) activity in yeast. Structural analysis of hybrid peptides reveals that an intact C-terminus and specific spacing are crucial for alpha-MF activity.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Background:

  • Saccharomyces cerevisiae alpha-mating factor (alpha-MF) shares structural homology with mammalian luteinizing hormone-releasing hormone (LHRH).
  • Previous studies indicated alpha-MF exhibits LHRH activity, but the converse was unconfirmed.

Purpose of the Study:

  • To investigate whether LHRH possesses alpha-MF activity in yeast.
  • To elucidate the structural determinants of alpha-MF and LHRH activities through synthesis and testing of hybrid peptides.

Main Methods:

  • Synthesis of hybrid peptides combining sequences of alpha-MF and LHRH.
  • Assay of alpha-MF and LHRH activities of synthesized peptides in yeast models.

Main Results:

Related Experiment Videos

  • LHRH did not exhibit alpha-MF activity in yeast.
  • The C-terminal tetrapeptide of alpha-MF was essential for its activity.
  • Insertion of a serine residue at position 4, as in LHRH, abolished alpha-MF activity.
  • Hybrid peptides displayed reduced LHRH activity compared to native hormones.

    • Conclusions:

    • Alpha-MF activity requires an intact C-terminus and precise spacing between N-terminal residues and the C-terminus.
    • Structural homology between alpha-MF and LHRH does not necessarily imply an evolutionary relationship.