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Effects of vitamin E on mammary and blood leukocyte function, with emphasis on chemotaxis, in periparturient dairy
I Politis1, N Hidiroglou, J H White
1Department of Animal and Food Sciences, University of Vermont, Burlington 05405, USA.
Objective:
To determine the effect of vitamin E supplementation on the immune system of dairy cows.
Design:
The following immune parameters were followed: production of chemotactic factors and superoxide by mammary macrophages and chemotactic responsiveness of blood neutrophils.
Animals:
16 healthy Holstein dairy cows.
Procedure:
Dairy cows were assigned to 1 of 2 experimental groups: control (no vitamin E supplementation) and vitamin E supplemented. Supplementation of vitamin E started 4 weeks before and continued up to 8 weeks after parturition, and included oral supplementation of vitamin E at the rate of 3,000 IU/cow/d. In addition, the same group of cows received 1 injection of vitamin E (5,000 IU) 1 week prior to the expected date of parturition. Blood samples were collected weekly throughout the experimental period.
Results:
Vitamin E supplementation enhanced by 30 to 83% (P < 0.05) chemotactic responsiveness of blood neutrophils beginning 2 weeks before to 4 weeks after parturition, compared with controls. There were no differences in production of superoxide or chemotactic factors by mammary macrophages between control and vitamin E-supplemented cows.
Conclusions:
Vitamin E supplementation prevents the periparturient inhibition of neutrophil chemotaxis. It is unlikely that vitamin E affects directly the function of mammary macrophages.