Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD34+Thy-1+Lin- stem cells from mobilized peripheral blood

L J Murray1, A Tsukamoto, R Hoffman

  • 1SyStemix Inc., Palo Alto, California 94304, USA.

Leukemia & Lymphoma
|June 1, 1996
PubMed
Summary

Mobilized peripheral blood (MPB) contains hematopoietic stem cells (HSCs) that can be purified for autologous transplantation. These CD34+Thy-1+Lin- HSCs offer a tumor-depleted graft and are targets for gene therapy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Oncologist and NHS Commissioner Perspectives on Reirradiation for Recurrent Cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Patient Perspectives on Reirradiation for Recurrent Cancer, to Inform Future Clinical Trial Design.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Response to 'Dose Accumulation for Pelvic Stereotactic Ablative Radiotherapy Reirradiation. In Regard to Slevin et al.'

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Dose Accumulation for Pelvic Stereotactic Ablative Radiotherapy Reirradiation.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Non-medicinal oral contrast in upper abdominal MRI for MR-guided radiotherapy: A scoping review.

Radiography (London, England : 1995)·2025
Same author

Phononic switching of magnetization by the ultrafast Barnett effect.

Nature·2024

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Transplantation Medicine

Background:

  • Mobilized peripheral blood (MPB) progenitor cells are increasingly used for autologous transplantation.
  • A subpopulation of CD34+Thy-1+Lineage (Lin)- cells within MPB exhibits enriched hematopoietic stem cell (HSC) activity.
  • Thy-1 expression is higher on CD34+ cells in some MPB samples compared to bone marrow (BM).

Purpose of the Study:

  • To describe the purification of CD34+Thy-1+Lin- HSCs from MPB for autologous transplantation.
  • To highlight the potential of these HSCs as a tumor-depleted graft for cancer patients.
  • To note the ongoing research into using this HSC population for gene transfer in HIV, cancer, and genetic disorders.

Main Methods:

  • Depletion of myeloid and erythroid cells from MPB leukapheresis samples.
  • High-speed cell sorting to purify CD34+Thy-1+Lin- HSCs.
  • Characterization of HSC activity within the purified cell population.

Main Results:

  • Sufficient numbers of CD34+Thy-1+Lin- HSCs can be purified from MPB for autograft use.
  • This HSC population demonstrates potential for a tumor-depleted autograft.
  • The CD34+Thy-1+Lin- HSCs are a focus for gene transfer strategies.

Conclusions:

  • Purified CD34+Thy-1+Lin- HSCs from MPB are a viable option for autologous transplantation.
  • These cells hold promise for cancer therapy by providing a tumor-depleted graft.
  • Further research is exploring gene therapy applications of these HSCs for various diseases.

Related Experiment Videos