Oxidative damage to lens membranes induced by metal-catalyzed systems
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Updated: Oct 7, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
M T Yugay1, P C Pereira, F Leiria
1Department of Ophthalmology and Visual Sciences, Coimbra University Hospitals, Portugal.
Abstract:
The oxidative effect of hydrogen peroxide, ascorbic acid and glucose in the presence of transition metals was studied. Incubation of bovine lens membranes with hydrogen peroxide/metal, ascorbic acid/metal and glucose/metal systems resulted in a significant augmentation of thiobarbituric acid-reactive substance content in the membranes. Presence of alpha-tocopherol decreased the extent of oxidative damage. This oxidation was found to be mediated by hydroxyl radicals. Dependence of hydroxyl radical generation upon the buffer used has been observed. In all experiments Cu(II) ions exhibited a higher efficiency compared to Fe(II) ions.
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