An activity of hydrolyzing elastase substrate succinyltrialanine p-nitroanilide in human bile

Human hepatic bile contained an activity of hydrolyzing N-succinyl-L-alanyl-Lalanyl-L-alanine-p-nitroanilide, a substrate for elastase. The activity was associated with high molecular weight materials and was metal dependent. Neither elastase nor elastase-alpha 2-macroglobulin complex was implicated in this activity.