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Related Experiment Videos

Single crystals of inulin

I André1, J L Putaux, H Chanzy

  • 1Centre de Recherches sur les Macromolécules Végétales (CNRS), Grenoble, France.

International Journal of Biological Macromolecules
|April 1, 1996
PubMed
Summary

Crystallographic analysis revealed two distinct inulin polymorphs, a hydrated orthorhombic form and a semi-hydrated pseudo-hexagonal form. Hydration levels, not inulin conformation, dictate the crystalline structure.

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Area of Science:

  • Carbohydrate Chemistry
  • Crystallography
  • Materials Science

Background:

  • Inulin, a polysaccharide, is known for its diverse biological roles and applications.
  • Understanding the crystalline structure of inulin is crucial for its industrial processing and functional properties.
  • Previous studies have suggested polymorphism in inulin, but detailed structural analysis under varying conditions is limited.

Purpose of the Study:

  • To investigate the crystalline structures of low molecular weight inulin under different hydration conditions.
  • To determine the unit cell parameters and space groups of inulin polymorphs.
  • To elucidate the relationship between hydration and inulin crystal structure.

Main Methods:

  • Growth of lamellar inulin crystals from aqueous ethanol solutions.

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  • Analysis using three-dimensional electron diffraction.
  • Characterization via X-ray powder diagrams.

    • Main Results:

    • Two crystalline polymorphs of inulin were identified: a hydrated orthorhombic form and a pseudo-hexagonal semi-hydrated form.
    • The hydrated form exhibited an orthorhombic unit cell (P2(1)2(1)2(1)) with specific dimensions.
    • The semi-hydrated form displayed pseudo-hexagonal symmetry with distinct unit cell parameters, indicating variations in water content rather than inulin conformation.

    Conclusions:

    • Inulin crystallizes in at least two distinct polymorphic forms dependent on hydration.
    • The observed structural differences are attributed to varying water content within the crystal lattice.
    • Inulin exhibits pseudo-hexagonal symmetry in its crystalline state, with a consistent advance per monomer along the chain axis.