Related Experiment Videos
Expression of poplar phenylalanine ammonia-lyase in insect cell cultures
G R McKegney1, S L Butland, D Theilmann
1Department of Plant Science, University of British Columbia, Vancouver, Canada.
Phytochemistry
|March 1, 1996
Abstract:
A cDNA encoding one of the phenylalanine ammonia-lyase genes from Populus trichocarpa x deltoides was inserted into a baculovirus expression vector and the PAL protein was successfully expressed in insect cell cultures. High levels of active holoenzyme were obtained that could be purified in a single chromatographic step. Site-directed mutagenesis and expression of the mutant enzyme confirmed that conversion of the putative active site serine202 residue to alanine is sufficient to destroy the catalytic activity of PAL.