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B-cell monoclonality in salivary lymphoepithelial lesions
R C Jordan1, E W Odell, P M Speight
1Department of Oral Pathology, Eastman Dental Institute for Oral Health Care Sciences, London, U.K.
European Journal of Cancer. Part B, Oral Oncology
|January 1, 1996
Summary
Most salivary gland lymphoepithelial lesions show B-cell monoclonality, indicating a high risk for lymphoma development. Polymerase chain reaction (PCR) is the most reliable method for detecting this B-cell monoclonality.
Area of Science:
- Pathology
- Oncology
- Immunology
Background:
- Lymphoma can originate in salivary gland lymphoepithelial lesions.
- Histological features are known, but initial lymphocyte clonality is unclear.
Purpose of the Study:
- To determine the prevalence of B-cell monoclonality in lymphoepithelial lesions.
- To compare the efficacy of different detection methods.
Main Methods:
- Examined 22 parotid gland lymphoepithelial lesions.
- Used polymerase chain reaction (PCR) for immunoglobulin heavy chain gene amplification.
- Employed in situ hybridization or immunohistochemistry for light chain restriction.
Main Results:
- B-cell monoclonality detected in 17/22 (77.3%) cases using combined methods.
- PCR identified monoclonal immunoglobulin heavy chain gene rearrangements in 15/22 (68%) cases.
- PCR demonstrated higher reliability than in situ hybridization or immunohistochemistry, likely due to fixation variations.
Conclusions:
- Salivary gland lymphoepithelial lesions frequently exhibit B-cell monoclonality.
- PCR is a more dependable technique for identifying B-cell monoclonality in these lesions compared to other methods.