A study of the influence of biphase calcium phosphate ceramics on bacterial strains: in vitro approach

An in vitro study of the influence of Biphase Calcium Phosphate Ceramics (BCPCs) on bacterial strains is presented. The test procedures were carried out in saline test solution to which different quantities (2,4,50,100, and 200 mg) of BCPCs(samples A and B) were added. The influence of BCPCs on standard bacterial strains has been determined by measuring bacterial contamination using the plate count method. The testing method was validated with membrane filtration and direct inoculation in culture media of sediment and supernatant. The test demonstrated reduction of the bacterial cell population in from 1 to 24 h in all experiments. The antibacterial effect revealed the specific inherent properties of the BCPCs under investigation.