Solid phase radioimmunoassay for quantitating anti-HBs in hepatitis B immune globulin

A solid phase radioimmunoassay was used to quantitate the antibody in hepatitis B immune globulin. Polystyrene tubes coated with HBsAg were incubated with several dilutions of trace-labeled immune globulin, and the specific uptake was measure. Resulting data could be used to obtain the total antibody concentration in micrograms per milliliter as well as the intrinsic binding constant and index of heterogeneity.