Related Experiment Videos
High-performance liquid chromatography of flufenamic acid in rat plasma
D Cerretani1, L Micheli, A I Fiaschi
1Institute of Pharmacology, University of Siena, Italy.
Journal of Chromatography. B, Biomedical Applications
|April 12, 1996
Abstract:
A simple high-performance liquid chromatographic (HPLC) method for the determination of flufenamic acid in rat plasma is described. After liquid-liquid extraction, the drug is separated by HPLC on a 5-microns octadecylsilica column (Nucleosil C18) with ultraviolet detection at 280 nm. Linear calibration graphs for flufenamic acid were constructed from 0.5 to 15 micrograms/ml. The method has been applied to a pharmacokinetic study in animals.