Sequence analysis and immunolocalisation of phosphatidylethanolamine binding protein (PBP) in human brain tissue

We have cloned and sequenced cDNA corresponding to human brain phosphatidylethanolamine binding protein (PBP) and have used the resultant data to generate PBP-specific anti-peptide antisera for immunohistochemical studies. The distribution of PBP was assessed immunohistochemically in sections from multiple regions of neonatal brain and spinal cord, including spinal nerve roots. Strong PBP immunoreactivity was present in the cytoplasm of oligodendrocytes in the white matter and Schwann cells in the nerve roots. There was only weak immunostaining within the neurones and neuropil. The findings are in keeping with a role for PBP in the organisation of phospholipids in the myelin sheath.