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Related Experiment Videos

Membrane-based, dry-reagent prothrombin time tests

S E Zweig1, B G Meyer, S Sharma

  • 1Avocet Medical, Inc., Los Gatos, CA 95032, USA.

Biomedical Instrumentation & Technology
|May 1, 1996
PubMed
Summary
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A new membrane-based assay offers a dry-reagent prothrombin time test for whole blood, simplifying coagulation analysis. This innovation provides accurate international normalized ratio (INR) results comparable to traditional methods.

Area of Science:

  • Biomedical Engineering
  • Clinical Chemistry
  • Hematology

Background:

  • Conventional prothrombin time (PT) assays often require liquid reagents and larger sample volumes.
  • Accurate monitoring of the extrinsic coagulation pathway is crucial for managing anticoagulant therapy.
  • There is a need for simplified, point-of-care coagulation testing methods.

Purpose of the Study:

  • To develop and validate a prototype membrane-based, dry-reagent prothrombin time assay for whole blood.
  • To assess the assay's performance using small sample volumes and evaluate its correlation with conventional methods.

Main Methods:

  • Utilized an asymmetric polysulfone membrane for plasma separation from whole blood (10 microliter samples).
  • Integrated calcium and thromboplastin within the membrane to facilitate extrinsic pathway reactions.

Related Experiment Videos

  • Monitored thrombin generation optically using a fluorescent substrate (rhodamine-110).
  • Analyzed fluorescence kinetics to derive a prothrombin time-equivalent parameter convertible to INR.

    • Main Results:

    • The membrane system successfully separated plasma and enabled complete extrinsic pathway reactions.
    • Optical monitoring of thrombin generation provided kinetic data for analysis.
    • Results demonstrated a strong correlation (R2 = 0.96) with conventional liquid phase prothrombin time assays.
    • The system is capable of converting prothrombin time results to international normalized ratio (INR) values.

    Conclusions:

    • The developed membrane-based, dry-reagent assay is a viable prototype for whole blood prothrombin time testing.
    • This system offers a simplified approach for coagulation analysis with potential for point-of-care applications.
    • The assay provides accurate INR values, comparable to established methods, using minimal sample volume.