Cloning of a gene encoding the precursor of nisin by PCR

The structural gene for the precursor of nisin was synthesized by polymerase chain reaction and then cloned in pUC18. The nucleotide sequence of the cloned precursor nisin gene was determined by dideoxy termination method. The sequence data obtained agreed with those of precursor nisin genes isolated by other workers from different Lactococcus lactis strains.