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The yeast SWISS-2DPAGE database

J C Sanchez1, O Golaz, S Frutiger

  • 1Clinical Chemistry Laboratory, Geneva Hospital, Switzerland. sanchez/dim.hcuge.CH

Electrophoresis
|March 1, 1996
PubMed
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Researchers created the yeast SWISS-2DPAGE database to map yeast proteins and understand gene functions. This resource aids in identifying protein changes related to biological processes.

Area of Science:

  • * Molecular Biology
  • * Genomics
  • * Proteomics

Background:

  • * The yeast genome sequencing is nearing completion, presenting a new challenge to understand gene functions.
  • * The European Functional Analysis (EUROFAN) project aims to elucidate the physiological and biochemical roles of yeast open reading frames (ORFs).
  • * Protein-based technologies are crucial for establishing a yeast reference map to identify functional changes.

Purpose of the Study:

  • * To describe the yeast SWISS-2DPAGE database for functional analysis of yeast proteins.
  • * To establish a comprehensive yeast reference map using protein identification techniques.
  • * To provide a publicly accessible resource for yeast proteome research.

Main Methods:

  • * Utilized two-dimensional gel electrophoresis with immobilized pH gradient (IPG) for charge separation.

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  • * Employed protein identification methods including gel comparison, amino acid composition analysis, and microsequencing.
  • * Recorded and described identified proteins in a standardized format within the SWISS-2DPAGE database.

    • Main Results:

    • * Identified and mapped over one hundred yeast polypeptides, including several novel ones.
    • * Developed the yeast SWISS-2DPAGE database as a central repository for proteomic data.
    • * Ensured the database is accessible via the World Wide Web (WWW) on the ExPASy server.

    Conclusions:

    • * The yeast SWISS-2DPAGE database is a valuable tool for functional genomics and proteomics research.
    • * This resource facilitates the identification of protein pattern changes linked to biological events.
    • * Free online access promotes collaborative research in yeast functional analysis.