Differential maturation of cholinergic interneurons in the striatal patch versus matrix compartments

Striatal neurons are generated in two distinct phases. Neurons that become postmitotic early in embryonic development come to be located primarily in the patch compartment of the striatum, while the majority of the neurons situated in the striatal matrix compartment are generated later in embryogenesis. The cholinergic interneurons in the striatum, which have been reported to be more or less homogeneously distributed in the adult, are all generated early in development. Given that early generated neurons are expected to be situated primarily in the patch compartment, we investigated the apparently homogeneous distribution of cholinergic neurons by analysing their localizations in the patch and matrix compartments during striatal development. To selectively mark the striatal patch compartment we made injections of the retrograde fluorescent tracer True Blue in the substantia nigra on embryonic day 20 or postnatal day (P)1, and then stained for cholineacetyltransferase (ChAT) at different time-points in development. After P7, the distribution of the ChAT positive neurons changes from an earlier preference for the patch compartment to a preference for an area of the matrix just outside of the patches. Absolute counts show that this change in distribution is caused mainly by a late turn on of ChAT by the cholinergic neurons in the matrix compartment. These data suggest that there are different compartmental subpopulations of cholinergic neurons in the striatum.