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Related Experiment Videos

Changes in the neuronal plasma membrane during synaptogenesis

L Surchev1, V Dontchev, K Ichev

  • 1Department of Anatomy and Histology, Faculty of Medicine, Sofia, Bulgaria.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|December 1, 1995
PubMed
Summary

Neuronal membranes mature during early development, increasing proteins and cholesterol domains to form functional synaptic membranes. This study tracks these key molecular changes in Wistar rats.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Synaptogenesis involves significant neuronal plasma membrane remodeling.
  • Understanding the molecular changes during this period is crucial for neuroscience.

Purpose of the Study:

  • To morphologically investigate in vivo changes of neuronal membrane components during early postnatal synaptogenesis.
  • To characterize the development of integral membrane proteins, cholesterol domains, carbohydrate residues, and anionic sites.

Main Methods:

  • Morphological analysis of rat cerebral cortex.
  • Preparation of growth cones (postnatal days 0-10) and synaptosomes (postnatal days 14-30).
  • Focus on integral membrane proteins, cholesterol, carbohydrate residues, and anionic sites.

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Main Results:

  • Early postnatal neuronal membranes show few intramembranous particles, small cholesterol domains, and scarce lectin-binding/anionic sites.
  • A progressive increase in these components was observed, leading to mature synaptic membranes.
  • Proteins and cholesterol molecules consistently occupied distinct membrane domains throughout development.

Conclusions:

  • Neuronal membrane maturation involves a significant increase in proteins and cholesterol domains.
  • This study elucidates the morphological basis for the development of functional synaptic membranes from immature precursors.