Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thiol compounds and organic nitrates

S Boesgaard1

  • 1Department of Medicine B, Rigshospitalet, Copenhagen.

Danish Medical Bulletin
|November 1, 1995
PubMed
Summary
This summary is machine-generated.

Organic nitrates, used for ischemic heart disease, can lose effectiveness over time. This study shows thiol compounds may help maintain nitrate effects by influencing their metabolism and counteracting side effects, potentially improving treatment outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized trial of low-dose, ultrasound-assisted thrombolysis or heparin for pulmonary embolism.

Cardiovascular research·2026
Same author

Hyponatraemia at hospital admission is a predictor of overall mortality.

Internal medicine journal·2014
Same author

The inflammatory biomarker YKL-40 at admission is a strong predictor of overall mortality.

Journal of internal medicine·2012
Same author

Diabetes mellitus and cardiogenic shock in acute myocardial infarction.

European journal of heart failure·2005
Same author

N-terminal proBNP and mortality in hospitalised patients with heart failure and preserved vs. reduced systolic function: data from the prospective Copenhagen Hospital Heart Failure Study (CHHF).

European journal of heart failure·2004
Same author

Cardiogenic shock complicating acute myocardial infarction; prognostic impact of early and late shock development.

European heart journal·2003
Same journal

Guidelines for Percutaneous Dilatational Tracheostomy (PDT) from the Danish Society of Intensive Care Medicine (DSIT) and the Danish Society of Anesthesiology and Intensive Care Medicine (DASAIM).

Danish medical bulletin·2011
Same journal

Pain and convalescence following laparoscopic ventral hernia repair.

Danish medical bulletin·2011
Same journal

Glucose-dependent Insulinotropic Polypeptide (GIP): From prohormone to actions in endocrine pancreas and adipose tissue.

Danish medical bulletin·2011
Same journal

Birth outcome in women with ulcerative colitis and Crohn's disease, and pharmacoepidemiological aspects of anti-inflammatory drug therapy.

Danish medical bulletin·2011
Same journal

Communication with patients and colleagues.

Danish medical bulletin·2011
Same journal

Short convalescence and minimal pain after out-patient Bascom's pit-pick operation.

Danish medical bulletin·2011
See all related articles

Area of Science:

  • Pharmacology
  • Cardiovascular Medicine
  • Biochemistry

Background:

  • Organic nitrates are crucial for treating ischemic heart disease.
  • Tolerance limits the therapeutic efficacy of continuous organic nitrate treatment.
  • The exact mechanisms of nitrate tolerance remain unclear, with thiol depletion being a leading theory.

Purpose of the Study:

  • To investigate in vivo thiol/nitrate interactions in various experimental and clinical settings.
  • To elucidate the role of intracellular thiols in organic nitrate metabolism and tolerance.
  • To explore how exogenous thiol administration affects nitrate efficacy and counter-regulatory mechanisms.

Main Methods:

  • Examined the hypotensive effects of nitroglycerin (NTG) in relation to intracellular glutathione (GSH) levels.

Related Experiment Videos

  • Compared arterial and venous thiol levels in nitrate-tolerant versus non-tolerant animals.
  • Assessed the impact of N-acetylcysteine (NAC) on nitrate effects and the renin-angiotensin system in rats and humans.

    • Main Results:

    • Reduced intracellular GSH levels diminished the acute hypotensive effect of NTG, highlighting GSH's role in nitrate metabolism.
    • Vascular thiol levels were similar in tolerant and non-tolerant animals, questioning thiol depletion as the sole cause of tolerance.
    • Exogenous NAC augmented NTG's hypotensive effect via a non-tolerance-specific mechanism, likely involving extracellular nitrate/thiol interaction and NO formation.
    • NAC inhibited angiotensin-converting enzyme, counteracting nitrate-induced renin-angiotensin system stimulation.
    • In clinical settings, NAC co-administration with isosorbide dinitrate (ISDN) delayed tolerance to antianginal effects and altered the vasodilator profile.

    Conclusions:

    • Cellular thiol levels significantly influence the hemodynamic effects of organic nitrates.
    • Nitrate tolerance may involve altered cellular thiol handling or related enzymes.
    • Exogenous thiols can potentiate nitrate effects through a distinct interaction and attenuate counter-regulatory responses, improving clinical outcomes.