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Fluorescent in situ hybridization on archival G-banded slides
1Department of Medical Genetics, University of Helsinki, Finland.
Cytogenetics and Cell Genetics
|January 1, 1996
Abstract:
We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 x SSC, Tween 20.