Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between Campylobacter jejuni and lipids

C M Szymanski1, G D Armstrong

  • 1Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.

Infection and Immunity
|September 1, 1996
PubMed
Summary

Campylobacter jejuni attachment to host cells primarily involves lipids, not carbohydrates. This bacterium binds best to intact lipids, especially those with unsaturated fatty acids, suggesting a complex interaction mechanism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complete Genome Sequence of Campylobacter jejuni Strain 12567, a Livestock-Associated Clade Representative.

Genome announcements·2018
Same author

Complete Genome Sequences of Three Campylobacter jejuni Phage-Propagating Strains.

Genome announcements·2018
Same author

Coadministration of the Campylobacter jejuni N-Glycan-Based Vaccine with Probiotics Improves Vaccine Performance in Broiler Chickens.

Applied and environmental microbiology·2017
Same author

Therapeutic potential of egg yolk antibodies for treating Clostridium difficile infection.

Journal of medical microbiology·2011
Same author

Carbohydrate receptor mimicry as a basis for antibacterial therapy.

Current opinion in drug discovery & development·2009
Same author

Glycan mimicry as a basis for novel anti-infective drugs.

Biochimie·2001

Area of Science:

  • Microbiology
  • Cell Biology
  • Biochemistry

Background:

  • Motility is crucial for Campylobacter jejuni pathogenesis and enhances bacterial attachment to host cells.
  • Understanding the molecular mechanisms of C. jejuni attachment is vital for controlling infections.

Purpose of the Study:

  • To investigate the role of carbohydrates and lipids in C. jejuni attachment to host epithelial cells.
  • To elucidate the specific types of lipids and their structural features involved in bacterial binding.

Main Methods:

  • Utilized Chinese hamster ovary (CHO) cell mutants with defects in complex carbohydrate biosynthesis.
  • Performed thin-layer chromatography overlay analysis with extracted lipids.
  • Employed receptor-based enzyme-linked immunosorbent assays to quantify bacterial-lipid interactions.

Main Results:

  • Carbohydrates, including simple sugars and complex oligosaccharides, play a minor role in C. jejuni attachment.
  • C. jejuni exhibits significant binding to lipids, with highest affinity for intact lipid structures.
  • Lipids containing unsaturated fatty acids showed the strongest binding affinity.
  • Hydrophobic interactions contribute minimally to the binding process.

Conclusions:

  • C. jejuni attachment to host cells involves interactions with lipids in cell membranes.
  • The binding affinity is dependent on the integrity and composition of the lipid structure.
  • Lipid interactions partially inhibit binding, indicating that multiple adhesion mechanisms are involved in C. jejuni pathogenesis.

Related Experiment Videos