Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The integrons In0, In2, and In5 are defective transposon derivatives

H J Brown1, H W Stokes, R M Hall

  • 1School of Biological Sciences, Macquarie University, New South Wales, Australia.

Journal of Bacteriology
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intermittent fasting 5:2 diet: What is the macronutrient and micronutrient intake and composition?

Clinical nutrition (Edinburgh, Scotland)·2020
Same author

Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549.

Biochemistry. Biokhimiia·2020
Same author

Neural cell responses to wear debris from metal-on-metal total disc replacements.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2019
Same author

Recovery rate data for silicon nitride nanoparticle isolation using sodium polytungstate density gradients.

Data in brief·2018
Same author

Structure of the K82 Capsular Polysaccharide from Acinetobacter baumannii LUH5534 Containing a d-Galactose 4,6-Pyruvic Acid Acetal.

Biochemistry. Biokhimiia·2018
Same author

Development and optimisation data of a tissue digestion method for the isolation of orthopaedic wear particles.

Data in brief·2018
Same journal

Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

Journal of bacteriology·2026
Same journal

Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

Journal of bacteriology·2026
Same journal

DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

Journal of bacteriology·2026
Same journal

Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

Journal of bacteriology·2026
Same journal

Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

Journal of bacteriology·2026
Same journal

YpeB stability affects germination possibly through delaying SleB activity in <i>Bacillus subtilis</i>.

Journal of bacteriology·2026
See all related articles

Class 1 integrons share similar structures, containing IS1326 insertion sequences. These defective transposon derivatives show evidence of IS1353 acquisition, impacting transposition capabilities and revealing evolutionary relationships.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Class 1 integrons are genetic elements found in various bacterial plasmids and transposons.
  • Integrons play a role in the acquisition and dissemination of antibiotic resistance genes.
  • Previous studies have characterized different types of integrons, including In0, In2, and In5.

Purpose of the Study:

  • To compare the structures of class 1 integrons In0, In2, and In5.
  • To investigate the role of insertion sequences IS1326 and IS1353 in integron evolution.
  • To understand the relationship between integrons and transposons, particularly mercury resistance transposons.

Main Methods:

  • Comparative analysis of DNA sequences of integrons In0, In2, and In5.
  • Identification and characterization of insertion sequences within the integrons.

Related Experiment Videos

  • Phylogenetic analysis to determine the evolutionary relationships of the integrons and associated transposons.

    • Main Results:

    • Class 1 integrons In0, In2, and In5 exhibit closely related structures.
    • All three integrons contain the insertion sequence IS1326, a novel member of the IS21 family.
    • IS1326 has led to deletions in adjacent sequences, resulting in defective transposon derivatives.
    • Integron In2 contains IS1353, an IS3 family member, acquired after integration into an ancestral mercury resistance transposon.

    Conclusions:

    • The structural similarities and presence of IS1326 suggest a common origin for In0, In2, and In5.
    • IS1326 and IS1353 have significantly shaped the structure and function of these integrons.
    • The findings provide insights into the evolution of class 1 integrons and their relationship with transposons, including mercury resistance elements.