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[Assignment of differentiation-relevant cDNA RA538 on human chromosomes by fluorescence in situ hybridization]
Zhonghua Yi Xue Za Zhi
|April 1, 1996
Abstract
Objective:
To localize the differentiation-relevant cDNA RA538 (3.8kb) onto the human chromosome 8q2.
Methods:
A modified non-isotopic labeling technique was used.
Results:
The cDNA RA538 was obtained by subtraction hybridization from human esophageal cancer cell line EC8712 induced by retinoic acid. Fluorescence in situ hybridization (FISH) using double-labeled probes showed clear and paired hybridization signals at the corresponding regions of both two chromatids in 18 out of 60 metaphases examined. While by single-labeling, only 7 were positive in 60 metaphases observed and the fluorescent spots were seen only at one chromatid.
Conclusion:
The modified FISH protocol is useful for mapping cDNA sequences of a few kilobases.