[Analysis of pathogenic features of mucoid Haemophilus influenzae infection in children]

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J L Liu1, M Deng1, C R Song1

  • 1Laboratory Center, Hunan Children's Hospital, Changsha 410007, China.

Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine]
|August 19, 2025
PubMed

Insights

Whole-genome sequencing identified 23 mucoid Haemophilus influenzae type b (Hib) strains in children. Analysis revealed dominant ST386 and ST18 types, highlighting the importance of Hib vaccination.

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Context:

  • Mucoid Haemophilus influenzae type b (Hib) infections pose a significant threat to children's health.
  • Understanding the genetic diversity and antimicrobial resistance patterns of circulating Hib strains is crucial for effective disease control.

Purpose:

  • To characterize the genetic makeup, virulence factors, and antimicrobial susceptibility of mucoid Hib strains isolated from children.
  • To identify dominant sequence types (STs) and population structures within the studied cohort.

Summary:

  • Whole-genome sequencing, MLST, and cgSNP analysis were performed on 23 mucoid Hib strains. All strains were identified as Hib, with ST386 and ST18 being the most prevalent. Virulence genes were universally present, while specific adhesion and metabolism genes were found in the novel ST2887. High resistance to Trimethoprim-sulfamethoxazole was observed.
  • Drug susceptibility testing revealed resistance to ampicillin, cefuroxime, and Trimethoprim-sulfamethoxazole, with sensitivity to other beta-lactam drugs.

Impact:

  • The findings underscore the continued circulation of Hib and the emergence of specific genetic lineages.
  • High resistance rates to Trimethoprim-sulfamethoxazole necessitate careful treatment strategies.
  • The study strongly advocates for continued and widespread Hib vaccination programs to prevent invasive disease.

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