Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The rat MHC haplotype RT1c expresses two classical class I molecules

E Joly1, L Leong, W J Coadwell

  • 1Department of Immunology, Babraham Institute, Cambridge, United Kingdom.

Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1996
PubMed
Summary

The RT1c haplotype in laboratory rats expresses two distinct classical class I molecules, RT1.A1c and RT1.A2c, challenging previous findings of only one molecule per haplotype. This discovery reveals two functional loci within the RT1.Ac region.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

2024 guidelines (S3) for the therapeutic management of patients with genital herpes.

Annales de dermatologie et de venereologie·2026
Same author

2024 recommendations for the therapeutic management of anogenital warts.

Annales de dermatologie et de venereologie·2026
Same author

Gram-negative toe web intertrigo associated with eczematous dermatoses improved with dupilumab: A case series.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same author

Global perspectives from women leaders in radiology: a view from five continents.

The British journal of radiology·2025
Same author

Treatment of vitiligo with intravenous bolus methylprednisolone followed by methotrexate and heliotherapy: A case series on La Reunion Island.

Annales de dermatologie et de venereologie·2025
Same author

Vitiligo treated with oral baricitinib and heliotherapy: A case series.

Annales de dermatologie et de venereologie·2024

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Rattus norvegicus research

Background:

  • Classical MHC class I coding sequences in Rattus norvegicus have been characterized for only four haplotypes.
  • Previous studies reported a single RT1.A classical class I molecule per haplotype.

Purpose of the Study:

  • To investigate the number of classical MHC class I molecules encoded by the RT1c haplotype in Rattus norvegicus.
  • To characterize the novel classical class I molecules found in the RT1c haplotype.

Main Methods:

  • Utilized recombinant rat strains to map serologic determinants.
  • Employed a PCR-based expression-cloning method to isolate functional cDNAs.
  • Characterized molecular species using a panel of RT1.Ac-reactive monoclonal antibodies (mAbs) and primary alloreactive cytotoxic T lymphocytes.

Related Experiment Videos

Main Results:

  • The RT1c haplotype expresses two distinct classical class I molecules, named RT1.A1c and RT1.A2c.
  • Allotypic determinants for both molecules map to the RT1.A region.
  • Monoclonal antibodies and cytotoxic T lymphocytes could distinguish between RT1.A1c and RT1.A2c, confirming them as distinct molecular species.

Conclusions:

  • The RT1.Ac region contains two distinct loci, each encoding a functional classical class I molecule.
  • This finding expands the known diversity of classical MHC class I molecules in Rattus norvegicus.