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Alternative processing of the sarco/endoplasmic reticulum Ca(2+)-ATPase transcripts during muscle differentiation is
L Van den Bosch1, L Mertens, Y Cavaloc
1Laboratory of Physiology, University of Leuven, Belgium.
The Biochemical Journal
|August 1, 1996
Summary
The muscle-specific SERCA2 isoform requires regulated splicing, not general efficiency changes. Specific splicing factors, not general ones like ASF/SF2, control this crucial process.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- The expression of the muscle-specific sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA2) isoform depends on a regulated splicing event.
- This specific splicing occurs at the 3'-end of the primary gene transcript, which is otherwise inefficient.
Purpose of the Study:
- To investigate the regulatory mechanisms governing SERCA2 splicing.
- To determine if SERCA2 splicing is controlled by general splice/polyadenylation efficiency or specific regulatory factors.
Main Methods:
- Analysis of SERCA2 splicing during B-cell maturation to assess changes in general splice and polyadenylation efficiency.
- Expression and overexpression studies of alternative splicing factors, including ASF/SF2.
Main Results:
- Changes in general splice and polyadenylation efficiency during B-cell maturation did not impact SERCA2 splicing.
- Expression levels of ASF/SF2 and other SR proteins were insufficient to regulate muscle- and neuronal-specific splicing.
Conclusions:
- SERCA2 splicing is a specifically regulated process, distinct from general changes in splicing or polyadenylation efficiency.
- The regulation of muscle- and neuronal-specific SERCA2 splicing is not mediated by alterations in ASF/SF2 or related proteins.