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Putrescine transport in differentiated Caco-2 cells
A M Barlier1, R Benamouzig, D Tomé
1Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, Paris.
Gastroenterologie Clinique Et Biologique
|March 1, 1996
Summary
Digestive polyamine transport involves oxidation. Putrescine uptake and transport in Caco-2 cells show an oxidative excreting pathway and a paracellular route, influenced by diamine oxidase.
Area of Science:
- Cell Biology
- Gastroenterology
- Biochemistry
Background:
- Polyamines are crucial in cellular functions.
- Understanding polyamine transport is vital for digestive health.
Purpose of the Study:
- To investigate the role of oxidation in putrescine uptake and transport.
- To evaluate polyamine importance in the digestive tract.
Main Methods:
- Utilized differentiated Caco-2 cell monolayers.
- Studied putrescine uptake and transport mechanisms.
- Investigated the effect of diamine oxidase inhibition.
Main Results:
- Confirmed putrescine uptake at both apical and basolateral membranes.
- Demonstrated efficient apical-to-basolateral putrescine transport and excretion.
- Identified a diamine oxidase-controlled oxidative catabolic pathway.
Conclusions:
- Suggests an oxidative pathway for putrescine excretion involving diamine oxidase.
- Indicates a paracellular route for putrescine transport.
- Oxidative excretion is inhibited by aminoguanidine.