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Increased serum laminin and angiogenin concentrations in patients with peripheral arterial occlusive disease
H Burgmann1, U Hollenstein, T Maca
1Department of Internal Medicine I, University of Vienna Medical School, Austria.
Insights
Serum laminin and angiogenin levels are elevated in peripheral arterial occlusive disease (PAOD). These findings suggest potential indicators of vascular damage in PAOD patients.
Area of Science:
- Vascular Biology
- Biomarkers
- Clinical Medicine
Background:
- Peripheral arterial occlusive disease (PAOD) significantly impacts patient mobility and quality of life.
- Understanding the molecular mechanisms underlying PAOD progression is crucial for developing effective treatments.
Purpose of the Study:
- To quantify serum laminin and angiogenin concentrations in patients across different stages of PAOD (Fontaine stages IIb, III, and IV).
- To compare these levels with those in healthy controls and patients with deep vein thrombosis.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to measure serum laminin and angiogenin.
- Study included patients with PAOD stages IIb, III, and IV, deep vein thrombosis patients, and matched healthy controls.
Main Results:
- Patients with stage IV PAOD exhibited significantly higher serum laminin and angiogenin concentrations compared to normal subjects and patients without PAOD.
- A notable correlation was observed between angiogenin and laminin, and between serum laminin and fibrinogen in stage IV PAOD patients.
Conclusions:
- Elevated serum laminin and angiogenin may serve as biomarkers for endothelial damage in PAOD.
- These increases could reflect reduced vascular perfusion or compensatory revascularization processes.
Aim:
To measure serum laminin and angiogenin concentrations in patients with peripheral arterial occlusive disease (PAOD) Fontaine stages IIb, III, and IV.
Methods:
The study population comprised 38 patients (20 men and 18 women) with stage IV PAOD, 11 patients (six men and five women) with stage III PAOD, 18 patients (10 men and eight women) with stage IIb PAOD, and 23 patients (10 men and 13 women) with deep vein thrombosis. Fifteen normal subjects (matched for risk factors) and 10 patients (five men and five women) without PAOD served as controls. Serum samples were obtained at admission and serum laminin and angiogenin concentrations were measured using an enzyme linked immunosorbent assay.
Results:
Patients with stage IV PAOD had higher serum laminin (mean +/- SEM; 826 +/- 97 ng/ml) and angiogenin concentrations (467 +/- 26 pg/ml) than normal subjects (laminin: 379 +/- 21 ng/ml; angiogenin: 358 +/- 16 pg/ml) and patients without PAOD (laminin: 277 +/- 34 ng/ml; angiogenin: 406 +/- 25 pg/ml). A significant correlation was found between angiogenin and laminin and between serum laminin and fibrinogen concentrations in patients with stage IV disease.
Conclusions:
Raised laminin and angiogenin concentrations may be indicators of endothelial damage caused by reduced vascular perfusion or compensatory revascularisation, or both.