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Primary structure of human plasma membrane Ca(2+)-ATPase isoform 3
B J Brown1, H Hilfiker, S J DeMarco
1Department of Biochemistry and Molecular Biology, Mayo Graduate School, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Biochimica Et Biophysica Acta
|August 14, 1996
Summary
Researchers determined the human plasma membrane calcium ATPase (PMCA) isoform 3 sequence. This isoform is exclusively found in brain tissue, providing complete sequence data for all human PMCA isoforms.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Plasma membrane calcium ATPase (PMCA) pumps are crucial for maintaining calcium homeostasis.
- Understanding different PMCA isoforms is essential for cellular function and disease research.
Purpose of the Study:
- To determine the complete coding sequence of the human PMCA isoform 3.
- To analyze the expression pattern of PMCA3 in human tissues.
Main Methods:
- Utilized overlapping genomic and cDNA clones for sequence determination.
- Employed Northern blot analysis to detect PMCA3 transcripts in various human tissues.
Main Results:
- The complete coding sequences for two major splice variants, PMCA3a and PMCA3b, were identified.
- A 7 kb PMCA3 transcript was exclusively detected in human brain tissue.
- Human PMCA3 isoforms show 98% identity to their rat counterparts.
Conclusions:
- The primary structure of human PMCA3 has been fully elucidated.
- PMCA3 expression is highly restricted to neuronal tissues in humans.
- Complete sequence information for all human PMCA isoforms is now available.