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Structural characterization of the exopolysaccharide produced by Lactobacillus acidophilus LMG9433
G W Robijn1, R Gutiérrez Gallego, D J van den Berg
1Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Netherlands.
Carbohydrate Research
|July 19, 1996
Abstract:
The exopolysaccharide produced by Lactobacillus acidophilus LMG9433 in a semi-defined medium was found to be a charged heteropolymer, with a composition of D-glucose, D-galactose, D-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose in molar ratios of 2:1:1:1. By means of methylation analysis, uronic acid degradation, de-N-acetylation/deamination, partial acid hydrolysis, and 1D/2D NMR studies the polysaccharide was demonstrated to consist of repeating units with the following structure: [Table: see text]