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The mouse immunoglobulin kappa locus contains about 140 variable gene segments
T Kirschbaum1, R Jaenichen, H G Zachau
1Adolf-Butenandt-Institut für Physiologische Chemie, Universität München, Germany.
European Journal of Immunology
|July 1, 1996
Summary
Researchers mapped the mouse immunoglobulin kappa locus using 46 yeast artificial chromosomes (YACs). This analysis identified 140 V kappa gene signals, contributing to a comprehensive understanding of the mouse immune system
Area of Science:
- Immunogenetics
- Molecular Biology
- Genomics
Background:
- The immunoglobulin kappa locus is crucial for adaptive immunity.
- Previous studies characterized some V kappa genes using cosmid libraries.
Purpose of the Study:
- To elucidate the complete structure of the mouse immunoglobulin kappa locus.
- To map V kappa, J kappa, and C kappa genes within the locus.
Main Methods:
- Analysis of 46 yeast artificial chromosomes (YACs) containing immunoglobulin kappa genes.
- Hybridization techniques using V kappa gene family-specific probes.
- Restriction fragment analysis (EcoRI) to characterize gene locations.
Main Results:
- Identified 140 V kappa gene signals across the analyzed YACs.
- Established a YACs panel representing the entire mouse kappa locus.
- Estimated the total mouse kappa locus size to be approximately 3.3 Mb.
Conclusions:
- The YACs panel provides a comprehensive map of the mouse immunoglobulin kappa locus.
- The study refines the understanding of V kappa gene organization and locus size.
- Identified V kappa genes likely located outside the kappa locus.