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Supravital staining of eosinophils
M Stankiewicz1, W Jonas, E Hadas
1AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. stankiewiczm@agresearch.cri.nz
International Journal for Parasitology
|April 1, 1996
Abstract:
Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.