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Proto-oncogene expression in human glomerular diseases

T Takemura1, M Okada, N Akano

  • 1Department of Pediatrics, Kinki University School of Medicine, Osaka, Japan.

The Journal of Pathology
|March 1, 1996
PubMed
Summary
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Proto-oncogene expression, including c-fos and c-myc, is elevated in glomerular cells of patients with proliferative glomerulonephritis and focal glomerular sclerosis. This expression correlates with disease severity and cellular proliferation.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Oncology

Background:

  • Proto-oncogenes like c-fos, c-myc, p53, and c-raf play roles in cell growth and differentiation.
  • Their dysregulation is implicated in various proliferative diseases, including certain kidney disorders.

Purpose of the Study:

  • To investigate the expression of c-fos, c-myc, p53, and c-raf in renal tissues from patients with glomerular diseases.
  • To correlate proto-oncogene expression with histological features and clinical parameters of glomerulonephritis.

Main Methods:

  • Immunofluorescent staining and in situ hybridization were used to detect protein and mRNA expression of c-fos, c-myc, p53, and c-raf in renal biopsy specimens.
  • Analysis included 73 patients with various glomerular diseases and normal controls.

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Main Results:

  • Elevated expression of c-Fos, c-Myc, p53 proteins, and c-Raf was observed in glomeruli of patients with proliferative glomerulonephritis (IgA nephritis, lupus nephritis) and focal glomerular sclerosis.
  • Higher numbers of proto-oncogene-positive glomerular cells were found in lupus nephritis, IgA nephritis, and focal segmental sclerosis compared to minimal change nephrotic syndrome and normal tissues.
  • In IgA nephritis, proto-oncogene expression correlated significantly with proliferating cell nuclear antigen (PCNA) positivity, mesangial hypercellularity, matrix expansion, and proteinuria levels.

Conclusions:

  • Proto-oncogene expression is associated with mesangial proliferation and matrix expansion in proliferative glomerulonephritis and focal glomerular sclerosis.
  • These findings suggest a role for proto-oncogenes in the pathogenesis of these kidney diseases.