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Related Experiment Videos

Adenosine nucleotides in bile

R S Chari1, S M Schutz, J E Haebig

  • 1Department of Surgery, Duke University, North Carolina, USA.

The American Journal of Physiology
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

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Adenosine nucleotides are present in bile and can activate purinergic receptors. This local nucleotide release may regulate biliary secretion through autocrine or paracrine signaling pathways.

Area of Science:

  • Hepatology
  • Cell Biology
  • Biochemistry

Background:

  • Purinergic receptors are activated by extracellular adenosine triphosphate (ATP).
  • Activation of these receptors stimulates chloride (Cl-) efflux in biliary epithelial cells.
  • The physiological presence and release of purinergic agonists in bile were previously uncharacterized.

Purpose of the Study:

  • To determine if purinergic agonists are present in mammalian bile under physiological conditions.
  • To assess the capability of hepatoma and cholangiocarcinoma cell lines for nucleotide release.
  • To investigate the potential role of local nucleotide release in modulating biliary secretion.

Main Methods:

  • Nucleotide concentrations in human, rat, and pig bile were measured using luminometry.

Related Experiment Videos

  • In vitro models of HTC rat hepatoma and Mz-ChA-1 cholangiocarcinoma cells were used to assess nucleotide release.
  • The effect of 5'-nucleotidase inhibition on AMP concentrations was evaluated.
  • Stimulation of nucleotide release by forskolin and ionomycin was examined in cell lines.

    • Main Results:

    • Adenosine nucleotides (ATP, ADP, AMP) were detected in mammalian bile, with significant concentrations in human bile (average 5.21 microM).
    • Both hepatoma and cholangiocarcinoma cell lines released nucleotides in vitro.
    • Inhibition of 5'-nucleotidase increased AMP levels, indicating ongoing nucleotide metabolism.
    • Cholangiocarcinoma cells, but not hepatoma cells, showed stimulated nucleotide release upon exposure to forskolin or ionomycin.

    Conclusions:

    • Mammalian bile contains adenosine nucleotides at concentrations sufficient to activate purinergic receptors.
    • Local release of nucleotides from biliary cells may function as an autocrine or paracrine mechanism.
    • This purinergic signaling could play a role in the regulation of biliary secretion.