Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

End points for biomonitoring: assay sensitivity/selectivity

C S Aaron1, D M Zimmer, P R Harbach

  • 1Upjohn Company, Kalamazoo, Michigan 49007, USA. saaron@pwinet.upj.com

Environmental Health Perspectives
|May 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Immunohistochemical expression of p53 with different clonal numbers in gastric adenocarcinoma and its significance].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2022
Same author

[Sensitization characteristics of Juniperus chinensis pollen in Beijing area].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2022
Same author

[Gene expression profile analysis involved in pathogenesis of allergic rhinitis by using bioinformatics method as a novel way of drug screening].

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery·2018
Same author

[Observation on pollinosis with vegetable food allergy].

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery·2018
Same author

[Clinical observation on specific immunotherapy of allergic rhinitis with artemisia pollen].

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery·2018
Same author

Veterinary and human biobanking practices: enhancing molecular sample integrity.

Veterinary pathology·2013
Same journal

A New Start.

Environmental health perspectives·2026
Same journal

Time-Varying Exposure to Element Mixtures and Children's Cognition at 5 Years of Age: Findings from the New Hampshire Birth Cohort Study.

Environmental health perspectives·2026
Same journal

Effect of Household Air Pollution on the Gut Microbiome and Virome of Adult Women Living in Uganda.

Environmental health perspectives·2026
Same journal

Comparison of Temperature-Mortality Associations across the Middle East Using Different Exposure Estimation Approaches.

Environmental health perspectives·2026
Same journal

Workflow for Statistical Analysis of Environmental Mixtures.

Environmental health perspectives·2026
Same journal

Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα.

Environmental health perspectives·2026
See all related articles

The hypoxanthine guanine phosphoribosyltransferase (hprt) mutation assay in cynomolgus monkeys shows similar mutation spectra to humans but lacks sensitivity for routine biomonitoring of general populations.

Area of Science:

  • Biomonitoring
  • Toxicology
  • Genetics

Background:

  • Biomonitoring estimates population exposure to hazards.
  • Assessing method sensitivity and linkage to human disease is crucial but often overlooked.
  • The hypoxanthine guanine phosphoribosyltransferase (hprt) gene is a common target for mutation detection.

Purpose of the Study:

  • To evaluate the sensitivity of hprt mutation measurement in cynomolgus monkeys.
  • To compare spontaneous mutation spectra between monkeys and humans.
  • To determine the suitability of the hprt assay for population biomonitoring.

Main Methods:

  • In vivo hprt mutation detection in cynomolgus monkeys.
  • Reverse transcriptase polymerase chain reaction (RT-PCR) for mutant sequencing.

Related Experiment Videos

  • Comparison of mutation spectra between species.

    • Main Results:

    • hprt mutations produced in vivo in monkeys were detected using a human-derived technique.
    • Spontaneous mutation spectra observed in monkeys showed similarity to humans.
    • The assay demonstrated low sensitivity, failing to detect potent mutagens and showing time-dependent patterns.

    Conclusions:

    • While hprt mutation analysis in cynomolgus monkeys mirrors human spontaneous mutation spectra, its low sensitivity limits its use in routine population biomonitoring.
    • Caution is advised when interpreting mutant frequency data due to assay limitations.
    • Archiving human samples may be valuable for future, more sensitive biomonitoring methods.