The S29 ribosomal protein increases tumor suppressor activity of K rev-1 gene on v-K ras-transformed NIH3T3 cells

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N Kondoh1, M Noda, R J Fisher

  • 1Department of Cell Genetics, Sasaki Institute, Tokyo, Japan.

Biochimica Et Biophysica Acta
|August 21, 1996
PubMed

Insights

Human S29 ribosomal protein (S29 rp) enhances Krev-1

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Colon carcinoma exhibits altered gene expression.
  • Ribosomal proteins play roles beyond translation.
  • Krev-1 protein is involved in cell growth regulation.

Purpose of the Study:

  • To investigate the role of human S29 ribosomal protein (S29 rp) in colon carcinoma.
  • To explore the functional relationship between S29 rp and Krev-1 protein.
  • To determine the contribution of S29 rp's zinc-binding domain.

Main Methods:

  • Differential hybridization screening of a colon carcinoma cDNA library.
  • Northern blot analysis to quantify mRNA levels.
  • Co-transfection assays in NIH3T3 cells with expression vectors.

Main Results:

  • S29 rp mRNA levels are higher in undifferentiated and rapidly proliferating HT29 colon cancer cells.
  • S29 rp mRNA levels inversely correlate with Krev-1 mRNA levels.
  • S29 rp significantly enhances the revertant-inducing activity of Krev-1(63E) in NIH3T3 cells.
  • S29 rp's zinc-binding domain is crucial for its interaction with Krev-1(63E).

Conclusions:

  • S29 ribosomal protein is implicated in colon cancer cell differentiation and proliferation.
  • S29 rp functionally interacts with Krev-1 protein, modulating its activity.
  • The zinc-binding activity of S29 rp is essential for its interaction with Krev-1.

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