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A constant current source for extracellular microiontophoresis

T Walker1, N Dillman, M L Weiss

  • 1Department of Computer and Electrical Engineering, Kansas State University, Manhattan 66506-5602, USA.

Journal of Neuroscience Methods
|December 1, 1995
PubMed
Summary
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A novel constant-current source facilitates extracellular microiontophoresis for tract-tracing substances. This reliable device offers precise current control and monitoring, enhancing experimental accuracy in neuroscience research.

Area of Science:

  • Neuroscience
  • Biomedical Engineering

Background:

  • Extracellular microiontophoresis is crucial for delivering tract-tracing substances in neuroscience.
  • Existing constant-current sources may lack precision and monitoring capabilities for demanding applications.

Purpose of the Study:

  • To describe a sophisticated constant-current source designed for extracellular microiontophoresis.
  • To highlight features enhancing reliability and accuracy in tract-tracing experiments.

Main Methods:

  • Utilized a flyback switched-mode power supply for high-voltage generation.
  • Incorporated operational amplifier circuitry for precise current regulation and instrumentation.
  • Implemented features for simultaneous load current and voltage monitoring, and adjustable current settings.

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Main Results:

  • Achieved a fast rise time with a stable +/- 2000 V supply (< 250 ms).
  • Enabled real-time monitoring of microelectrode resistance via an output voltage monitor.
  • Included voltage-limit indicator circuitry to signal inability to deliver desired current (e.g., blocked micropipette).
  • Ensured delivery of +/- 20 microA current through 100 M omega resistance with a high-compliance power supply.

Conclusions:

  • The developed constant-current source is a reliable tool for extracellular microiontophoresis.
  • Its features, including real-time monitoring and high compliance, improve experimental control and success rates for tract-tracing studies.