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Updated: Aug 9, 2026

A Method of Nodose Ganglia Injection in Sprague-Dawley Rat
Published on: November 25, 2014
Non-adrenergic non-cholinergic nerve-mediated inhibitory control of pigeon oesophageal muscle
F Bonvissuto1, T Vetri, R Fileccia
1Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi di Palermo, Italia.
Abstract:
Pigeon oesophageal smooth muscle in vitro has spontaneous electromechanical activity. In the presence of atropine and guanethidine, electrical field stimulation evokes a transient TTX-sensitive response comprising inhibition of electric bursting activity and muscular relaxation. This NANC inhibitory response was analysed using the K+ channel blockers TEA and apamin, TEA perfusion (0.1-5 mM) induced a concentration-dependent reduction in amplitude of EFS-evoked relaxation. Responses to higher stimulation frequencies were more sensitive to TEA than those to lower ones. The maximum reduction in amplitude (29% of control) was obtained on 30 Hz EFS evoked responses during 5 mM TEA perfusion. In a similar way, apamin (0.01-10 microM) perfusion reduced NANC relaxation, up to 30% of control. These results suggest that in the pigeon oesophagus, NANC intramural neurons are responsible for muscular relaxation. We speculate that an increase in K+ conductance might be the main mechanism involved, although the residual response after K+ channel blockade indicates the existence of an additional ionic mechanism.
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