Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineering enzymes for stability

A Shaw1, R Bott

  • 1Genencor International, 925 Page Mill Road, Palo Alto, CA 94304, USA.

Current Opinion in Structural Biology
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using kidney transplantation to explore epigenetic markers of frailty.

GeroScience·2026
Same author

Dyrk1a inhibition with the Novel Compound DYR533: A Cross-Disease Therapeutic Strategy Targeting Amyloidosis, Tau Pathogenesis, and Neuroinflammation.

bioRxiv : the preprint server for biology·2026
Same author

Exertional heat illness: employment outcomes following military heat tolerance assessment.

Occupational medicine (Oxford, England)·2025
Same author

What are the greatest opportunities for innovation to improve access to and quality of palliative care services to children? A qualitative interview study.

BMC palliative care·2025
Same author

Thrombosis with thrombocytopenia syndrome (TTS) following adenovirus vector COVID-19 vaccination in Canada.

Vaccine·2023
Same author

iRefer: past, present and future.

Clinical radiology·2022
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

Enzyme stabilization strategies are improving, moving beyond simple rules of thumb. Understanding factors affecting protein folding and unfolding kinetics is key to achieving reliable enzyme engineering for enhanced stability.

Area of Science:

  • Biochemistry
  • Protein Engineering
  • Enzyme Kinetics

Background:

  • Recent advancements focus on enzyme stabilization through methods like stabilizing folded states, destabilizing unfolded states, and modifying unfolding kinetics.
  • Current approaches often rely on empirical rules of thumb rather than established engineering principles.
  • Enzyme stability encompasses both thermodynamic stability (reversible denaturation) and kinetic stability (unfolding rates).

Purpose of the Study:

  • To explore the factors influencing enzyme stability, including those affecting folded and unfolded states.
  • To investigate the role of folding and unfolding kinetics in maintaining enzyme stability.
  • To enhance the understanding of protein stabilization through site-specific substitutions and their impact on transition-state free energies.

Related Experiment Videos

Main Methods:

  • Analysis of factors affecting the stability of folded versus unfolded protein states.
  • Assessment of factors influencing the rates of protein folding and unfolding.
  • Examination of site-specific substitutions and their effect on transition-state free energies.

Main Results:

  • Identified key factors influencing both thermodynamic and kinetic stability of enzymes.
  • Demonstrated that understanding kinetics and transition-state free energies is crucial for predicting stabilization effects.
  • Highlighted the need for moving beyond empirical rules towards predictive principles in enzyme engineering.

Conclusions:

  • Enzyme stabilization requires a comprehensive understanding of factors governing protein folding, unfolding, and kinetics.
  • Site-specific substitutions impacting transition-state free energies are critical for measurable protein stabilization.
  • Future enzyme engineering efforts should focus on developing reliable principles for predictable stabilization.