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Related Experiment Videos

[Chromatographic support to remove solvent/detergent mixture utilized as viral attenuation agent]

L Guerrier1, I Flayeux, E Boschetti

  • 1BioSepra SA, Villeneuve-la-Garenne, France.

Annales Pharmaceutiques Francaises
|January 1, 1996
PubMed
Summary

A novel sorbent efficiently removes virus inactivating solvent-detergent mixtures from biological fluids. This method allows for high-capacity treatment in small columns, enabling efficient purification of large sample volumes.

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Materials Science

Context:

  • Solvent-detergent (SD) mixtures are widely used for virus inactivation in biological products.
  • Residual SD agents can be toxic and interfere with downstream processes.
  • Efficient removal of these agents from large volumes of biological fluids is challenging.

Purpose:

  • To describe a novel sorbent material and method for the specific capture of solvent-detergent mixtures.
  • To demonstrate the high capacity and efficiency of the sorbent for treating biological fluids.
  • To present a cost-effective and scalable purification solution.

Summary:

  • A specialized sorbent has been developed for the selective adsorption of virus inactivating solvent-detergent mixtures.

Related Experiment Videos

  • The sorbent exhibits high capacity, allowing treatment of large fluid volumes using small columns (sorbent volume is 1/10 to 1/4 of sample volume).
  • The sorbent can be repeatedly used after classical cleaning/sanitization or sterilization with strong oxidizing agents.

    • Impact:

    • Enables efficient and safe purification of large batches of biological products.
    • Reduces the risk of toxicity and interference from residual inactivation agents.
    • Offers a scalable and potentially cost-effective solution for biopharmaceutical manufacturing.