Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue kallikreins in evolutionarily diverse vertebrates

G P Richards1, J Chao, L Chao

  • 1National Marine Fisheries Service, Southeast Fisheries Science Center, Charleston Laboratory, SC 29422, USA.

Immunopharmacology
|May 1, 1996
PubMed
Summary

Three novel tissue kallikreins were discovered in lower vertebrates, including a mole, frog, and sea bass. These acidic enzymes were characterized and compared to mammalian kallikreins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Cluster-Randomized Clinical Trial of Silver Diamine Fluoride vs Atraumatic Restoration in Older Adults.

Journal of dental research·2026
Same author

Comparative efficacy of PD-1 blockade in patients with dMMR/MSI-H metastatic colorectal or gastric cancer: a global retrospective study.

ESMO gastrointestinal oncology·2026
Same author

Skin pain and sleep quality numeric rating scales for children aged 6 months to 5 years with atopic dermatitis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Margetuximab with retifanlimab as first-line therapy in HER2+/PD-L1+ unresectable or metastatic gastroesophageal adenocarcinoma: MAHOGANY cohort A.

ESMO open·2022
Same author

[Differential on N6-methyladenosine modification of circRNA in early inflammation of silicosis].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2022
Same author

Predictive role of microsatellite instability for PD-1 blockade in patients with advanced gastric cancer: a meta-analysis of randomized clinical trials.

ESMO open·2021

Area of Science:

  • Biochemistry
  • Comparative Physiology

Background:

  • Tissue kallikreins are serine proteases involved in various physiological processes.
  • Previous research has primarily focused on mammalian kallikreins.

Purpose of the Study:

  • To discover and characterize novel tissue kallikreins in lower vertebrates.
  • To compare the biochemical and functional properties of these novel kallikreins with their mammalian counterparts.

Main Methods:

  • Isolation and purification of kallikreins from the salivary gland of Scalopus aquaticus, Rana berlandieri pancreas, and Centropristis striata skeletal muscle.
  • Biochemical characterization including molecular mass, isoelectric point (pI), and susceptibility to inhibitors.
  • Functional assays involving kininogen cleavage and kinin peptide release.

Related Experiment Videos

Main Results:

  • Discovery of three novel kallikreins from mole, frog, and sea bass.
  • Characterization revealed acidic proteins (pI 4.2-5.3) with molecular masses of 27-36 kDa.
  • Sea bass prokallikrein activation by trypsin was demonstrated.
  • Comparative analysis showed similarities and differences in immunoreactivity, physicochemical properties, and kininogen cleavage compared to human and rat kallikreins.

Conclusions:

  • The study expands the known diversity of tissue kallikreins across vertebrate species.
  • Novel kallikreins from lower vertebrates possess distinct biochemical properties and functional activities.
  • These findings provide insights into the evolution of the kallikrein-kinin system.