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Effect of Pasteurella multocida vaccination on buffalo polymorphonuclear hydrogen peroxide and nitric oxide
1Division of Biochemistry and Food Science, Indian Veterinary Research Institute, Izatnagar, India.
Abstract:
An attempt was made to investigate the effect of Pasteurella multocida on certain microbicidal reactive oxygen and nitrogen intermediates released by the polymorphonuclear cells (PMNs) from the vaccinated animals. The PMNs from the peripheral blood of both control and experimental buffaloes vaccinated against haemorrhagic septicaemia were isolated. PMNs from control animals upon activation with P. multocida lipopolysaccharide (LPS) and live P. multocida cells generated higher levels of hydrogen peroxide (H2O2) and nitric oxide (NO-) than the non-activated cells (P < 0.01). In the presence of P. multocida LPS, PMNs from animals vaccinated against haemorrhagic septicaemia generated significantly higher H2O2 (P < 0.05) and NO- (P < 0.01) than the PMNs from control animals. L-Arginine when added to the activation medium enhanced the production of NO- in a dose-dependent manner. This indicated the role of arginine in NO- production. The study suggested that buffalo PMNs possessed a potent oxidant defence system even in the presence of P. multocida, an antiphagocytic bacterium.
Insights
Vaccinated buffaloes
Area of Science:
- Veterinary Immunology
- Bacterial Pathogenesis
- Cellular Microbiology
Background:
- Haemorrhagic septicaemia, caused by Pasteurella multocida, is a significant threat to buffalo health.
- Polymorphonuclear cells (PMNs) are crucial in combating bacterial infections through the release of reactive oxygen and nitrogen intermediates.
- Understanding the host-pathogen interaction at the cellular level is vital for developing effective vaccines.
Purpose of the Study:
- To investigate the impact of Pasteurella multocida on microbicidal reactive oxygen and nitrogen intermediates produced by buffalo PMNs.
- To compare the immune response of PMNs from vaccinated versus control buffaloes when exposed to P. multocida.
- To explore the role of L-arginine in nitric oxide production by buffalo PMNs.
Main Methods:
- Isolation of peripheral blood polymorphonuclear cells (PMNs) from control and vaccinated buffaloes.
- Activation of PMNs using Pasteurella multocida lipopolysaccharide (LPS) and live P. multocida cells.
- Quantification of hydrogen peroxide (H2O2) and nitric oxide (NO-) production by activated PMNs.
- Assessment of L-arginine's effect on NO- production.
Main Results:
- Activated PMNs from control buffaloes produced significantly higher levels of H2O2 and NO- compared to non-activated cells.
- PMNs from vaccinated buffaloes exhibited enhanced H2O2 and NO- production in response to P. multocida LPS compared to control PMNs.
- L-arginine supplementation dose-dependently increased NO- production, confirming its role in the pathway.
Conclusions:
- Buffalo PMNs possess a robust oxidant defense system capable of responding effectively to Pasteurella multocida.
- Vaccination against haemorrhagic septicaemia enhances the microbicidal activity of buffalo PMNs.
- The findings suggest that the arginine-nitric oxide pathway is important in the innate immune response of buffaloes to P. multocida.