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Modulation of osteoblast behaviour by tenascin
1Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK.
Journal of Cell Science
|June 1, 1996
Summary
Tenascin, an extracellular matrix protein, stimulates osteoblast differentiation and maintains their function. Blocking tenascin reduces alkaline phosphatase and collagen synthesis, indicating its crucial role in bone cell behavior.
Area of Science:
- Biochemistry
- Cell Biology
- Bone Biology
Background:
- Tenascin is an extracellular matrix protein secreted by osteoblasts.
- Tenascin is absent from the mineralized bone matrix.
- The role of tenascin in osteoblast behavior is not fully understood.
Purpose of the Study:
- To investigate the role of tenascin in osteoblast morphology, differentiation, and proliferation.
- To test the hypothesis that tenascin regulates osteoblast behavior.
Main Methods:
- Utilized three osteoblast-like cell lines (UMR-106, ROS-17/2.8, SAOS-2).
- Assessed cell adhesion, alkaline phosphatase activity, and collagen synthesis.
- Investigated the effect of anti-tenascin antibodies and cytochalasin D on cell behavior.
Main Results:
- Two cell lines specifically adhered to tenascin, remaining rounded.
- Tenascin stimulated alkaline phosphatase activity in two of three cell lines.
- Anti-tenascin reduced alkaline phosphatase and inhibited collagen synthesis.
- Anti-tenascin affected cell proliferation differently across cell lines, suggesting tenascin's inhibitory role in some contexts.
Conclusions:
- Tenascin stimulates osteoblastic differentiation.
- Endogenous tenascin maintains the functional state of cultured osteoblast-like cells.
- Tenascin plays a significant role in regulating osteoblast behavior.