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Related Experiment Videos

Quantifying cell mono-layer cultures by video imaging

K S Miller1, L A Hook

  • 1University of Tulsa, OK 74104, USA. millerks@centum.utulsa.edu

Biotechniques
|April 1, 1996
PubMed
Summary

This study presents a sensitive and reproducible method for quantifying adherent cells in multi-well plates using Giemsa staining and digital imaging. This technique is valuable for in vitro cell number and proliferation studies.

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Area of Science:

  • Cell biology
  • Biotechnology
  • Microscopy

Context:

  • Accurate quantification of adherent cells is crucial for in vitro studies.
  • Traditional methods can be labor-intensive and prone to variability.
  • There is a need for reliable and efficient cell counting techniques.

Purpose:

  • To describe a novel method for assaying the relative number of adherent cells.
  • To utilize Giemsa staining, video imaging, and software for quantification.
  • To establish a sensitive and reproducible cell assay.

Summary:

  • A method is detailed for quantifying adherent cells in multi-well plates.
  • Cells are stained with Giemsa and imaged using a video camera and CCD.
  • Image analysis software quantifies the relative cell number.

Impact:

  • Provides a sensitive and reproducible assay for in vitro cell quantification.
  • Facilitates studies requiring measurement of relative cell numbers.
  • Supports research on cell proliferation and drug screening.

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