Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

As MAD as can be

R J Read1

  • 1Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.

Structure (London, England : 1993)
|January 15, 1996
PubMed
Summary
This summary is machine-generated.

Mutiwavelength anomalous dispersion (MAD) now provides accurate experimental phases for high-resolution protein studies. This technique aids in investigating protein solvation and flexibility, and advancing crystallographic methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular pathology of X linked retinoschisis: mutations interfere with retinoschisin secretion and oligomerisation.

The British journal of ophthalmology·2005
Same author

Pushing the boundaries of molecular replacement with maximum likelihood.

Acta crystallographica. Section D, Biological crystallography·2001
Same author

Different structural requirements for plasminogen activator inhibitor 1 (PAI-1) during latency transition and proteinase inhibition as evidenced by phage-displayed hypermutated PAI-1 libraries.

Journal of molecular biology·2001
Same author

Immunoprophylactic potential of cloned Shiga toxin 2 B subunit.

The Journal of infectious diseases·2001
Same author

Structure of a serpin-protease complex shows inhibition by deformation.

Nature·2000
Same author

Crystal structure of Pseudomonas aeruginosa PAK pilin suggests a main-chain-dominated mode of receptor binding.

Journal of molecular biology·2000
Same journal

Meet the author: Stephen Brohawn.

Structure (London, England : 1993)·2026
Same journal

Tetraspanins bring Norrin into focus: Structural insights into ligand-specific Wnt signaling.

Structure (London, England : 1993)·2026
Same journal

Uncovering subtype-selective activation of the K<sub>Ca</sub>3.1 channel by SKA-111.

Structure (London, England : 1993)·2026
Same journal

Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.

Structure (London, England : 1993)·2026
Same journal

Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states.

Structure (London, England : 1993)·2026
Same journal

Multifaceted effects of N-glycosylation on amyloidogenic κ light chains in AL amyloidosis.

Structure (London, England : 1993)·2026
See all related articles

Area of Science:

  • Structural biology
  • Biophysics

Background:

  • Accurate experimental phasing is crucial for determining protein structures.
  • Traditional methods have limitations in resolving protein dynamics and solvent interactions.

Purpose of the Study:

  • To highlight the utility of multiwavelength anomalous dispersion (MAD) for high-resolution protein crystallography.
  • To showcase MAD as a tool for studying protein solvation and flexibility.
  • To present MAD as a platform for developing new crystallographic techniques.

Main Methods:

  • Mutiwavelength anomalous dispersion (MAD) phasing.
  • High-resolution X-ray crystallography.

Main Results:

  • Demonstrated accurate experimental phase determination using MAD.

Related Experiment Videos

  • Achieved high-resolution structural data.

    • Conclusions:

    • MAD is a powerful tool for high-resolution protein structure determination.
    • MAD facilitates the study of protein solvation and flexibility.
    • MAD serves as a valuable test-bed for crystallographic method development.