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[Evaluation of 3 culture media for isolating mycobacteria from extrapulmonary samples]
J Esteban1, M L Valero-Moratalla, M Jiménez-Arriero
1Servicio de Microbiología Médica, Fundación Jiménez Díaz, Madrid.
Objective:
To compare three different media (Middlebrook 7H11, Löwenstein-Jensen and MB-Check System) for the isolation of mycobacteria from extrapulmonary samples in a routine medium-size microbiology laboratory.
Material And Methods:
Extrapulmonary samples were processed for mycobacterial isolation during a 22-month period, and the three media above described were evaluated in paralell during the usual routine work.
Results:
907 extrapulmonary specimens were processed during the study period, being the MB-Check system the most sensitive medium for detection of mycobacterial growth (79.3%) compared with Middlebrook 7H11 (62.2%) and Löwenstein-Jensen (71.8%), increasing the overall sensitivity in 11.1%. However, the fastest medium for detection of mycobacterial growth was Middlebrook 7H11 (18.2 days) compared with Löwenstein-Jensen (21.6 days) and MB-Check (23.8 days), despite the higher rates of contamination detected in Middlebrook 7H11 (61.4% compared with 10.8% for Löwenstein-Jensen and 6.1% for MB-Check).
Conclusions:
The combination of the three studied media is a useful alternative for isolation of mycobacteria from extrapulmonary specimens in medium-size laboratories.