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Performance evaluation of the FACSCount System: a dedicated system for clinical cellular analysis
K Strauss1, I Hannet, S Engels
1Becton Dickinson Immunocytometry Systems Europe, Erembodegem, Belgium.
Cytometry
|March 15, 1996
Summary
The FACSCount System offers accurate CD4, CD8, and CD3 absolute counts using flow cytometry for clinical labs. This dedicated instrument ensures reliable results for patient management with minimal sample handling.
Area of Science:
- Clinical laboratory diagnostics
- Immunophenotyping
- Cellular analysis technology
Background:
- Flow cytometers provide multiparameter cellular data, crucial for biological insights.
- Dedicated instruments are needed to adapt flow cytometry for routine clinical use.
Purpose of the Study:
- To introduce the FACSCount System as a dedicated flow cytometer for clinical laboratories.
- To highlight the system's capabilities in providing absolute counts of specific immune cells.
Main Methods:
- Utilizes flow cytometry to determine CD4, CD8, and CD3 absolute counts.
- Employs minimal sample handling and lysis-free cell preparation.
- Features automated fluorescence gating and calibrated reference beads for accuracy.
Main Results:
- The FACSCount System provides absolute counts of CD4, CD8, and CD3 from 100 microliters of whole blood.
- Quality control measures ensure accuracy, precision, and reproducibility of CD4 counts.
Conclusions:
- The FACSCount System is optimized for clinical settings, simplifying flow cytometry procedures.
- Ensures reliable CD4 counts essential for patient monitoring and management.