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Countertranscript-driven attenuation system of the pAM beta 1 repE gene
E Le Chatelier1, S D Ehrlich, L Jannière
1Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Molecular Microbiology
|June 1, 1996
Summary
Replication of the pAM beta 1 plasmid requires the RepE protein. Its gene transcription is reduced by a CopF repressor and further diminished by a novel countertranscript-driven attenuation system.
Area of Science:
- Molecular Biology
- Plasmid Replication
- Bacterial Genetics
Background:
- The RepE protein is essential for pAM beta 1 plasmid replication.
- Plasmid pAM beta 1 originates from Enterococcus faecalis.
- RepE gene transcription is negatively regulated by the CopF repressor.
Purpose of the Study:
- To investigate the transcriptional regulation of the RepE gene.
- To identify additional regulatory mechanisms controlling pAM beta 1 plasmid replication.
Main Methods:
- Extensive mutagenesis of the pAM beta 1 plasmid.
- Analysis of gene transcription and regulation.
Main Results:
- A countertranscript-driven transcriptional attenuation system further reduces RepE gene transcription by approximately 10-fold.
- This system operates via a mechanism similar to that of plasmid pT181's repC gene.
Conclusions:
- Plasmid pAM beta 1 replication is subject to dual negative regulation.
- A novel countertranscript-mediated attenuation system significantly impacts RepE expression.