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Cloning of Murine T Cells
Gaur1
1Department of Medicine, Stanford University Medical Center, Stanford, California, 94305
Methods (San Diego, Calif.)
|June 1, 1996
Summary
Researchers developed a method to create long-term, antigen-specific cultures of murine T cells, focusing on CD4(+) T-cell clones for studying mammalian immune responses.
Area of Science:
- Immunology
- Cellular Biology
Background:
- T-cell clones are crucial for understanding cellular immunity in mammals.
- Previous methods may lack efficiency or specificity in generating T-cell cultures.
Purpose of the Study:
- To present a reliable method for generating homogeneous, long-term, antigen-specific murine T-cell cultures.
- To provide guidance on obtaining CD4(+) T-cell clones specifically.
Main Methods:
- Culture of murine T cells under specific conditions to ensure antigen specificity and long-term viability.
- Focus on protocols for isolating and expanding CD4(+) T-cell populations.
- Inclusion of characterization assays for T-cell clones.
Main Results:
- Successful generation of homogeneous, long-term cultures of antigen-specific murine T cells.
- Demonstrated efficacy in obtaining CD4(+) T-cell clones.
- Established useful assays for characterizing these T-cell clones.
Conclusions:
- The described method provides a valuable tool for immunological research.
- This technique facilitates in-depth study of the cellular immune response using specific T-cell clones.
