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Allele assignment for HLA-A, -B, and -C genes to the Tenth International Histocompatibility Workshop cell lines
1Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, USA. v-prasad@skimskec.org
Abstract:
Development of DNA typing for Class I HLA alleles has lagged behind that of class II for a variety of technical reasons. Following the recognition of locus specific sequences in the first and the third intron, and acquiring the ability to amplify genomic DNA by intron-based PCR primer, we have devised DNA typing of class I alleles by SSOP and direct sequencing. In this study using these techniques we provide the allelic typing of HLA-A, -B, and -C genes for the B-lymphoblastoid reference cell lines from the Tenth International Histocompatibility Workshop. We also describe some common associations of the C alleles with HLA-A and HLA-B alleles.