Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sensitivity of cell lines to mitomycin C

P L Watts1, J A Plumb, J M Courtney

  • 1Bioengineering Unit, University of Strathclyde, Glasgow, UK.

British Journal of Urology
|March 1, 1996
PubMed
Summary

The cytotoxicity of mitomycin C increases in acidic conditions, impacting its effectiveness in treating bladder carcinomas. Adjusting the pH of chemotherapy vehicles may improve treatment outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating the potential of polymer nanoparticles for oral delivery of paclitaxel in drug-resistant cancer.

Cancer nanotechnology·2015
Same author

Effects of an internal transcription unit and its orientation on retrovirus titre and expression.

Cytotechnology·2012
Same author

Candidate DNA methylation drivers of acquired cisplatin resistance in ovarian cancer identified by methylome and expression profiling.

Oncogene·2012
Same author

Cell sensitivity assays : clonogenic assay.

Methods in molecular medicine·2011
Same author

Cell sensitivity assays : the MTT assay.

Methods in molecular medicine·2011
Same author

Pharmacokinetic and pharmacodynamic properties of an oral formulation of the histone deacetylase inhibitor Belinostat (PXD101).

Cancer chemotherapy and pharmacology·2010

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Mitomycin C is a chemotherapy agent used to treat various cancers.
  • The efficacy of chemotherapy drugs can be influenced by physiological factors such as pH.

Purpose of the Study:

  • To investigate the impact of varying pH levels on the cytotoxic effects of mitomycin C.
  • To assess the relationship between pH and mitomycin C's effectiveness across different cell lines.

Main Methods:

  • Utilized five established cell lines (three human, two mouse) for in vitro testing.
  • Exposed cell cultures to a range of mitomycin C concentrations (320 pM–50 µM) at different pH levels (5.8–7.8) for 2 hours.
  • Quantified cytotoxicity using a tetrazolium-dye-based microtitration assay.

Main Results:

  • Mitomycin C demonstrated significantly greater cytotoxicity in acidic conditions (pH 5.8–6.0) compared to neutral to alkaline pH (7.2–7.8) in three of the five cell lines.
  • A similar, though less pronounced, trend was observed in the remaining two cell lines.
  • These findings indicate a pH-dependent activity of mitomycin C.

Conclusions:

  • The cytotoxicity of mitomycin C is enhanced under acidic conditions for all tested cell lines.
  • The pH of the vehicle used in intravesical chemotherapy for bladder carcinomas is a critical factor for treatment success.
  • Urinary pH variations may contribute to the observed differences in patient treatment outcomes with intravesical mitomycin C therapy.

Related Experiment Videos